879 FRUCTOSE-1.6-DIPHOSPHATASE ACTIVITY IN HUMAN TERM PLACENTA
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چکیده
منابع مشابه
Crystalline Alkaline Form Fructose-1,6=diphosphatase
A simple and rapid procedure has been found for the preparation of highly pure alkaline form fructose-1,6-diphosphatase from turkey liver. The protein is tetrameric and is composed of subunits comprised of a 29,000 molecular weight core fragment and an associated 7,000-dalton Speptide. Large single crystals of the protein, suitable for xray diffraction analysis, are produced in the course of th...
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Previous studies on the carbohydrate metabolism of neoplastic liver have established definite qualitative and quant i ta t ive alterations in the glucose-6-phosphate (G-6-P) utilization of this tumor. The G-6-P metabolism was examined in homogenates and supernatantfluid of Novikoff hepatoma and control tissues by studying the behavior of enzymes directly involved in the utilization of this hexo...
متن کاملPARTIAL PURIFICATION AND PROPERTIES OF L-GLUTAMINE: D-FRUCTOSE 6-P AMIDOTRANSFERASE FROM HUMAN PLACENTA
The first enzyme of the pathway for uridine diphosphate N-acetyl-D-glucosamine (UDPAG) biosynthesis i.e. L-glutamine: D-fructose 6-P amidotransferase (E.C. 2.6.1.16) was purified 52-fold from human placenta using methanol fractionation and column chromatography on DEAE-Sephadex A-50. The enzyme showed optimal activity in a broad range of pH from 5.8 to 7.8 in both phosphate and cacodylate ...
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1. The properties of fructose diphosphatase from liver of South American lungfish (Lepidosiren paradoxa) were examined. 2. Saturation curves for substrate (fructose diphosphate) and both cofactors (Mn(2+) and Mg(2+)) are sigmoidal and Hill plots of these results suggest about 2 interacting substrate and cofactor sites/molecule of enzyme. 3. Mn(2+) is an efficient positive modulator of the enzym...
متن کاملTemperature and the regulation of enzyme activity in poikilotherms. Properties of rainbow-trout fructose diphosphatase.
1. The properties of fructose diphosphatase from the liver of rainbow trout (Salmo gairdnerii) were examined over the physiological temperature range of the organism. 2. Saturation curves for substrate (fructose 1,6-diphosphate) and a cofactor (Mg(2+)) are sigmoidal, and Hill plots of the results suggest a minimum of two interacting fructose 1,6-diphosphate sites and two interacting Mg(2+) site...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1978
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-197804001-00884